Thyroglobulin ELISA 试剂盒
Quick Overview for Thyroglobulin ELISA 试剂盒 (ABIN930684)
抗原
See all Thyroglobulin (TG) ELISA试剂盒适用
检测方法
实验类型
应用范围
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Analytical Method
- Quantitative
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产品特性
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ELISA kit for the detection of Thyroglobulin in the research laboratory
Alternative Names: Thyroglobulin ELISA kit
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Thyroglobulin (TG) ELISA Kit
TG 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Thyroglobulin (TG) ELISA KitTG 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Thyroglobulin (TG) ELISA KitTG 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thyroglobulin (TG) ELISA KitTG 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Thyroglobulin (TG) ELISA KitTG 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Thyroglobulin (TG) ELISA KitTG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Thyroglobulin (TG) ELISA KitTG 适用: 小鼠 Colorimetric Competition ELISA 10-160 ng/mL Plasma, Serum, Tissue Homogenate
Thyroglobulin (TG) ELISA KitTG 适用: 大鼠 Colorimetric Competition ELISA 10-160 ng/mL Plasma, Serum, Tissue Homogenate
Thyroglobulin (TG) ELISA KitTG 适用: Pig Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
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应用备注
- Optimal conditions to be determined by end-user
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板类型
- Pre-coated
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实验流程
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Highly purified human thyroglobulin (TG) is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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- Thyroglobulin (TG)
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别名
- Thyroglobulin
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物质类
- Hormone
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背景
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Thyroglobulin (hTg), a water soluble glycoprotein with a molecular weight of approx. 660,000 Da, is the principal constituent of the thyroidal colloid sharing about 75 % of it's mass. Synthesis of the thyroid hormones T3 and T4 is based on the oxidative iodination of thyrosine residues of the thyroglobulin molecule. Within the cell thyroglobulin is transported by the microsomes. Together with the secretion of T3 and T4 also small amounts of hTg are liberated into circulation.
Synonyms: Thyroglobulin ELISA kit. -
途径
- Thyroid Hormone Synthesis
抗原 See all Thyroglobulin (TG) ELISA试剂盒
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