GABA ELISA 试剂盒
Quick Overview for GABA ELISA 试剂盒 (ABIN930657)
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See all GABA ELISA试剂盒适用
实验类型
应用范围
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产品特性
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ELISA kit for the detection of GABA in the research laboratory
Alternative Names: GABA ELISA kit
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gamma-Aminobutyric Acid (GABA) ELISA Kit
GABA 适用: Various Species Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
gamma-Aminobutyric Acid (GABA) ELISA KitGABA 适用: 大鼠 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
gamma-Aminobutyric Acid (GABA) ELISA KitGABA 适用: 小鼠 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
gamma-Aminobutyric Acid (GABA) ELISA KitGABA 适用: 人 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
gamma-Aminobutyric Acid (GABA) ELISA KitGABA 适用: 豚鼠 Colorimetric Competition ELISA 100-2500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
gamma-Aminobutyric Acid (GABA) ELISA KitGABA 适用: 大鼠 Colorimetric Competition ELISA 100-2500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal conditions to be determined by end user
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实验流程
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This competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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- GABA (gamma-Aminobutyric Acid (GABA))
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别名
- GABA
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物质类
- Amino Acid
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背景
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Gamma-Aminobutyric acid (GABA) is the chief inhibitory neurotransmitter in the mammalian central nervous system. It plays a role in regulating neuronal excitability throughout the nervous system. In humans, GABA is also directly responsible for the regulation of muscle tone. In vertebrates, GABA acts at inhibitory synapses in the brain by binding to specific transmembrane receptors in the plasma membrane of both pre and postsynaptic neuronal processes.
Synonyms: GABA ELISA kit.
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