Estradiol ELISA 试剂盒
(1 reference)Quick Overview for Estradiol ELISA 试剂盒 (ABIN930631)
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实验类型
应用范围
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产品特性
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ELISA kit for the detection of Estradiol in the research laboratory
Alternative Names: Estradiol ELISA kit -
纯化方法
- Antiserum
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Estradiol ELISA Kit
适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: 绵羊, Cow, 马 Colorimetric Competition ELISA 9.37 pg/mL - 600 pg/mL Plasma, Serum
Estradiol ELISA Kit适用: 大鼠, Pig Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum
Estradiol ELISA Kit适用: 人, 猴, 小鼠 Colorimetric Competition ELISA 9.37 pg/mL - 600 pg/mL Plasma, Saliva, Serum, Urine
Estradiol ELISA Kit适用: Cow Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Estradiol ELISA KitSmall Sample 适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estradiol ELISA KitInstant 适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: Various Species Colorimetric Sandwich ELISA Fecal, Tissue Culture Medium, Urine
Estradiol ELISA Kit适用: 绵羊 Colorimetric Competition ELISA 40-1400 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal conditions to be determined by end user
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实验流程
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This Salivary Estradiol ELISA kit is based on the competition principle and the microplate separation. An unknown amount of Estradiol present in the sample and a fixed amount of Estradiol conjugated with horseradish peroxidase compete for the binding sites of a polyclonal Estradiol antiserum coated onto the wells. After two hours incubation the microtiter plate is washed to stop the competition reaction. Having added the substrate solution the concentration of Estradiol is inversely proportional to the optical density measured.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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: "Local and humoral immune responses against primary and repeat Neisseria gonorrhoeae genital tract infections of 17beta-estradiol-treated mice." in: Vaccine, Vol. 26, Issue 45, pp. 5741-51, (2008) (PubMed).
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: "Local and humoral immune responses against primary and repeat Neisseria gonorrhoeae genital tract infections of 17beta-estradiol-treated mice." in: Vaccine, Vol. 26, Issue 45, pp. 5741-51, (2008) (PubMed).
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- Estradiol
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物质类
- Hormone
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背景
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Estradiol (1,3,5(10)estratriene3,17 beta-diol, 17 beta-estradiol, E21) is a C18 steroid hormone with a phenolic A ring. This steroid hormone has a molecular weight of 272.4. It is the most potent natural Estrogen, produced mainly by the Graffian follicle of the female ovary and the placenta, and in smaller amounts by the adrenals, and the male testes. Estradiol (E2) is secreted into the blood stream where 98 % of it circulates bound to sex hormone binding globulin (SHBG) and to a lesser extent to other serum proteins such as albumin. Only a small fraction circulates as free hormone or in the conjugated form. Estrogenic activity is effected via estradiol receptor complexes which trigger the appropriate response at the nuclear level in the target sites. These sites include the follicles, uterus, breast, vagina, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin. In nonpregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation. The rising estradiol concentration is understood to exert a positive feedback influence at the level of the pituitary where it influences the secretion of the gonadotropins, follicle stimulating hormone (FSH), and luteinizing hormone (LH), which are essential for follicular maturation and ovulation, respectively. Following ovulation, estradiol levels fall rapidly until the luteal cells become active resulting in a secondary gentle rise and plateau of estradiol in the luteal phase. During pregnancy, maternal Estradiol levels increase considerably, to well above the preovulatory peak levels and high levels are sustained throughout pregnancy.
Synonyms: Estradiol ELISA kit.
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