Osteocalcin ELISA 试剂盒
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- 抗原 See all Osteocalcin (BGLAP) ELISA试剂盒
- Osteocalcin (BGLAP)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- Analytical Method
- Quantitative
- 产品特性
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ELISA kit for the detection of Osteocalcin in the research laboratory
Alternative Names: Osteocalcin ELISA kit - 纯化方法
- Purified human PBL T cells
- Top Product
- Discover our top product BGLAP ELISA Kit
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Osteocalcin (BGLAP) ELISA Kit
BGLAP 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 小鼠 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 小鸡 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 pg/mL - 20 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Osteocalcin (BGLAP) ELISA KitBGLAP 适用: 豚鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal conditions to be determined by end user
- 板类型
- Pre-coated
- 实验流程
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This osteocalcin assay is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiter plates. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of human osteocalcin. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1-human osteocalcin-MAb 2-HRP, the microtiter plate is washed to remove unbound enzyme labelled antibody. Bound enzymelabelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiter plate is then read at the appropriate wavelength. The amount of substrate turnover is determined colorimetrically by measuring the absorbance, which is proportional to the osteocalcin concentration. A calibration curve is plotted and OST concentration in samples is determined by interpolation from the calibration curve. The use of the EASIA reader (linearity up to 3 OD units) and a sophisticated data reduction method (polychromatic data reduction) result in a high sensitivity in the low range and in an extended calibration range.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原 See all Osteocalcin (BGLAP) ELISA试剂盒
- Osteocalcin (BGLAP)
- 别名
- Osteocalcin (BGLAP 产品)
- 背景
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Osteocalcin or bone Gla protein (B.G.P) is the major non-collagen protein of the bone matrix. It has a molecular weight of 5800 Da and contains 49 amino acids, including 3 residues of gamma carboxyl glutamic acid. Osteocalcin is synthesized in the bone by the osteoblasts. After production, it is partly incorporated in the bone matrix and the rest is found in the blood circulation. The exact physiological function of osteocalcin is still unclear. A large number of studies show that the circulating levels of osteocalcin reflect the rate of bone formation.
Synonyms: human Osteocalcin ELISA kit.
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