CA 19-9 ELISA 试剂盒
Quick Overview for CA 19-9 ELISA 试剂盒 (ABIN930405)
抗原
See all CA 19-9 ELISA试剂盒适用
检测方法
实验类型
应用范围
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Analytical Method
- Quantitative
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产品特性
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ELISA kit for the detection of CA 199 in the research laboratory
Alternative Names: CA 19-9 ELISA kit
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Gastrointestinal Cancer Antigen CA19-9 (CA 19-9) ELISA Kit
CA 19-9 适用: 人 Colorimetric Sandwich ELISA 6.25 IU/mL - 400 IU/mL Cell Culture Supernatant, Plasma, Serum
Gastrointestinal Cancer Antigen CA19-9 (CA 19-9) ELISA KitCA 19-9 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 IU/mL - 40 IU/mL Plasma, Serum, Tissue Homogenate
Gastrointestinal Cancer Antigen CA19-9 (CA 19-9) ELISA KitCA 19-9 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 IU/mL - 40 IU/mL Plasma, Serum, Tissue Homogenate
Gastrointestinal Cancer Antigen CA19-9 (CA 19-9) ELISA KitCA 19-9 适用: 人 Colorimetric Sandwich ELISA 3.125 IU/mL - 200 IU/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal conditions to be determined by end user
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板类型
- Pre-coated
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实验流程
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The CA 19-9 ELISA test is based on the principle of a solid phase enzymelinked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact CA 19-9 molecule is used for solid phase immobilization (on the microtiter wells). Another CA 19-9 monoclonal antibody conjugated to horseradish peroxidase (HRP) is in the antibodyenzyme conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the CA 19-9 molecules being sandwiched between the solid phase and enzymelinked antibodies. After two separate incubation steps at 37 °C for 90 minutes, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution ch anging the color to yellow. The concentration of CA 19-9 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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- CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))
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别名
- CA 19-9
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物质类
- Disease
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背景
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A group of mucin type glycoprotein Sialosyl Lewis Antigens (SLA), such as CA 19-9 and CA 19-5, have come to be recognized as circulating cancer associated antigens for gastrointestinal cancer. CA 19-9 represents the most important and basic carbohydrate tumor marker. The immunohistologic distribution of CA 19-9 in tissues is consistent with the quantitative determination of higher CA 19-9 concentrations in cancer than in normal or inflamed tissues. Recently reports indicates that the serum CA 19-9 level is frequently elevated in the serum of subjects with various gastrointestinal malignancies, such as pancreatic, colorectal, gastric and hepatic carcinomas. Together with CEA, elevated CA 19-9 is suggestive of gall-bladder neoplasm in the setting of inflammatory gallbladder disease. This tumor-associated antigen may also be elevated in some non-malignant conditions. Research studies demonstrate that serum CA 19-9 values may have utility in monitoring subjects with these diagnosed malignancies. It has been shown that a persistent elevation in serum CA 19-9 value following treatment may be indicative of occult metastatic and/or residual disease. A persistently rising serum CA 19-9 value may be associated with progressive malignant disease and poor therapeutic response. A declining CA 19-9 value may be indicative of a favorable prognosis and good response to treatment.
Synonyms: CA 19-9 ELISA kit.
抗原 See all CA 19-9 ELISA试剂盒
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