LH ELISA 试剂盒
适用: 激素
Colorimetric
Sandwich ELISA
-
- 抗原
- LH
- 适用
- 激素
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- Analytical Method
- Quantitative
- 产品特性
-
ELISA kit for the detection of LH in the research laboratory
Alternative Names: LH ELISA kit, Luteinizing Hormone ELISA kit
-
-
- 应用备注
- Optimal conditions to be determined by end user
- 板类型
- Pre-coated
- 实验流程
-
The LH ELISA kit is a solid phase direct sandwich method. The samples and diluted antiLHHRP conjugate are added to the wells coated with Mab to LH beta subunit. LH in the patient's serum binds to antiLH MAb on the well and the antiLH second antibody then binds to LH. Unbound protein and HRP conjugate are washed off by wash buffer. Upon theaddition of the substrate, the intensity of color is proportional to the concentration of LH in the samples. A standardcurve is prepared relating color intensity to the concentration of the LH.
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
-
- 抗原
- LH
- 物质类
- Hormone
- 背景
-
Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LHRH or GnRH), that is released by the hypothalamus. LH, also called interstitial cell stimulating hormone (ICSH) in men, is a glycoprotein with a molecular weight of approximately 30,000 Da. It is composed of two noncovalently associated dissimilar amino acid chains, alpha and beta. The alpha chain is similar to that found in human thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and human chorionic gonadotropin (hCG). LH stimulates ovulation and ovarian steroid production in the female. In the male, LH controls Leydig cell secretion of testosterone. LH is elevated in the Luteal phase of the menstrual cycle, primary hypogonadism, Gonadotropin secreting pituitary tumors and menopause. LH is deceased in hypothalamic GnRH deficiency, pituitary LH deficiency and ectopic steroid production.
Synonyms: LH ELISA kit, Luteinizing Hormone ELISA kit.
-
You are here:
