Gastrin I ELISA 试剂盒
适用: 人
Competition ELISA
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- 抗原
- Gastrin I
- 适用
- 人
- 实验类型
- Competition ELISA
- 应用范围
- ELISA
- 产品特性
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ELISA kit for the detection of Gastrin I in the research laboratory
Alternative Names: Human Gastrin I ELISA kit
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- 应用备注
- Optimal conditions to be determined by end user
- 实验流程
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This human Gastrin I kit is a competitive immunoassay for the determination of Gastrin in samples. The kit uses a polyclonal antibody to Gastrin I to bind, in a competitive manner, the Gastrin in the sample or an alkaline phosphatase molecule which has Gastrin I covalently attached to it. After a simultaneous incubation at roomtemperature the excessreagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Gastrin I in either standards or samples. Themeasured optical density is used to calculate the concentration of Gastrin I.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C.
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- 抗原
- Gastrin I
- 背景
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Gastrin is synthesized as a 101 residue prepropeptide on the rough endoplasmic reticulum, then post-translationally modified by cleavage and alpha-amidation to result in the active forms G34, G17 and G13/14, Big, Little and Mini-Gastrins respectively. Other forms also exist, but are not considered biologically significant. There are two types of G17 and G34, type II is sulfated at the tyrosine-12 residue, while type I is not. Both G34 and G17 circulate and contribute to the stimulation of gastric acid secretion, but have different clearance rates. In man, G17 has a circulating halflife of about 9 minutes while G34 has a halflife of about 35 minutes. G34 is the major circulating gastrin in fasting serum, but with G17, increases two to threefold after feeding until both are present in approximately equal amounts. Fasting gastrin concentration is usually less than 30 pmol/L (equivalent to 62.9 pg/mL) while patients with Zollinger-Ellison Syndrome and gastrinomas have levels exceeding 1,000 pg/mL.
Synonyms: Human Gastrin I ELISA kit.
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