IgG1 ELISA 试剂盒
Quick Overview for IgG1 ELISA 试剂盒 (ABIN930364)
抗原
See all IgG1 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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Analytical Method
- Quantitative
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产品特性
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ELISA kit for the detection of Mouse IgG1 Isotyping in the research laboratory
Alternative Names: Mouse IgG1 Isotyping ELISA kit -
纯化方法
- Protein A affinity column
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IgG1 ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG1 ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 617.28 ng/mL - 50000 ng/mL Plasma, Serum
IgG1 ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 200 ng/mL Plasma, Serum, Urine
IgG1 ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
IgG1 ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
IgG1 ELISA Kit适用: 绵羊 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
IgG1 ELISA Kit适用: 豚鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
IgG1 ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 49.4 ng/mL - 4000 ng/mL Plasma, Serum
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Mouse IgG1 Isotyping ELISA kit
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板类型
- Pre-coated
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实验流程
- This mouse IgG1 Isotyping Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of mouse IgG1 in Tissue Culture Media and serum. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to mouse IgG immobilized on a microtiter plate to bind the mouse IgG in the standards or sample. A mouse IgG1 Standard is provided in thekit. After a simultaneous incubation with a polyclonal antibody to mouse IgG1 conjugated to Horseradish peroxidase, which binds to the mouse IgG1 captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. Themeasured optical density is directly proportional to the concentration ofmouse IgG in either standards or samples. - See more at: https://www.fitzgerald-fii.com/mouse-igg1-isotyping-elisa-kit-55r-ib09602.html#sthash.qbaaakZ4.dpuf
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实验流程
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This mouse IgG1 Isotyping Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of mouse IgG1 in Tissue Culture Media and serum.
Please read the complete kit insert before performing this assay.
The kit uses a polyclonal antibody to mouse IgG immobilized on a microtiter plate to bind the mouse IgG in the standards or sample. A mouse IgG1 Standard is provided in the kit.
>After a simultaneous incubation with a polyclonal antibody to mouse IgG1 conjugated to Horseradish peroxidase, which binds to the mouse IgG1 captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse IgG in either standards or samples. -
限制
- 仅限研究用
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C
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- IgG1
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物质类
- Antibody
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背景
- IgG is divided into four subclasses, IgG1, IgG2, IgG3, and IgG4. IgG is the most abundant immunoglobulin found in the blood. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a varying portion called the "hinge region" which is unique to each IgG. This region allows for molecular flexibility and sets IgG1 apart from its IgG counterparts. IgG1 properties and functions include neutralization, opsonization, activation of the complement system, diffusion into extravascular sites and crossing the placenta. Synonyms: Mouse IgG1 Isotyping ELISA kit.
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