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HAVCR1 ELISA 试剂盒

Name: HAVCR1 ELISA 试剂盒
SKU: ABIN921117
Availability: OutOfStock

This 人 HAVCR1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 HAVCR1. This ELISA Kit has been cited in 3+ publications.

ELISA

1 image

(3 references)

产品编号 ABIN921117

发货至: 中国

Quick Overview for HAVCR1 ELISA 试剂盒 (ABIN921117)

抗原

See all HAVCR1 ELISA试剂盒

HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

抗原表位

AA 21-288

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2-2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Urine

Discover our top product HAVCR1 ELISA Kit

最低检测浓度

31.2 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KIM1

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: S21-T288

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<2pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: S21-T288
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA Kit
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Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA Kit
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HAVCR1 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate, Urine

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Belongs to the immunoglobulin superfamily. TIM family.
Tissue Specificity: Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. .

板类型

Pre-coated

实验流程

human KIM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KIM1 has been precoated onto 96-well plates. Standards(NSO, S21-T288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KIM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KIM1 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human KIM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KIM1 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 253, Standard deviation: 11.64, CV(%): 4.6
- Sample 2: n=16, Mean(pg/ml): 746, Standard deviation: 38.8, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 1220, Standard deviation: 74.42, CV(%): 6.1,
- Sample 1: n=24, Mean(pg/ml): 317, Standard deviation: 20.3, CV(%): 6.4
- Sample 2: n=24, Mean(pg/ml): 882, Standard deviation: 59.1, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 1458, Standard deviation: 115.2, CV(%): 7.9
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Lei, Li, Zeng, Mu, Yang, Zhou, Wang, Zhang: "Value of urinary KIM-1 and NGAL combined with serum Cys C for predicting acute kidney injury secondary to decompensated cirrhosis." in: Scientific reports, Vol. 8, Issue 1, pp. 7962, (2018) (PubMed).

Benli, Ayyildiz, Cirrik, Noyan, Ayyildiz, Cirakoglu: "Early term effect of ureterorenoscopy (URS) on the Kidney: research measuring NGAL, KIM-1, FABP and CYS C levels in urine." in: International braz j urol : official journal of the Brazilian Society of Urology, Vol. 43, Issue 5, pp. 887-895, (2017) (PubMed).

Dai, Wang, Lin, Zhang, Wang: "Renoprotective effects of berberine as adjuvant therapy for hypertensive patients with type 2 diabetes mellitus: Evaluation via biochemical markers and color Doppler ultrasonography." in: Experimental and therapeutic medicine, Vol. 10, Issue 3, pp. 869-876, (2015) (PubMed).
抗原 See all HAVCR1 ELISA试剂盒

HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

别名

HAVCR1

物质类

Virus

背景

Protein Function: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. .
Background: KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rats is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to Rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the Rat Tim1 gene and database analysis, the HAVCR1 gene was mapped to 5q33.2.
Synonyms: Hepatitis A virus cellular receptor 1,HAVcr-1,Kidney injury molecule 1,KIM-1,T-cell immunoglobulin and mucin domain-containing protein 1,TIMD-1,T-cell immunoglobulin mucin receptor 1,TIM,TIM-1,T-cell membrane protein 1,HAVCR1,KIM1, TIM1, TIMD1,
Full Gene Name: Hepatitis A virus cellular receptor 1
Cellular Localisation: Membrane, Single-pass type I membrane protein.

基因ID

26762

UniProt

Q96D42