HAVCR1 ELISA 试剂盒
Quick Overview for HAVCR1 ELISA 试剂盒 (ABIN921114)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse KIM1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: Y22-T212 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <2pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: Y22-T212
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Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA Kit
HAVCR1 适用: 小鼠 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Tissue Homogenate, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Tissue Homogenate, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 小鼠 Colorimetric Sandwich ELISA 0.078 ng/mL - 5 ng/mL Plasma, Serum, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse KIM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for KIM1 has been precoated onto 96-well plates. Standards(NSO, Y22-T212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KIM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse KIM1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse KIM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, tissue homogenates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse KIM1 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 306, Standard deviation: 12.24, CV(%): 4
- Sample 2: n=16, Mean(pg/ml): 640, Standard deviation: 35.2, CV(%): 5.5
- Sample 3: n=16, Mean(pg/ml): 1118, Standard deviation: 69.32, CV(%): 6.2,
- Sample 1: n=24, Mean(pg/ml): 418, Standard deviation: 24.7, CV(%): 5.9
- Sample 2: n=24, Mean(pg/ml): 825, Standard deviation: 58.6, CV(%): 7.1
- Sample 3: n=24, Mean(pg/ml): 1263, Standard deviation: 96, CV(%): 7.6
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
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别名
- HAVCR1
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物质类
- Virus
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背景
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Background: KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al.(1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al.(2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al.(2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
Synonyms: Hepatitis A virus cellular receptor 1 homolog ,Hepatitis A virus cellular receptor 1, isoform CRA_b ,Havcr1 ,mCG_117142 ,
Full Gene Name: hepatitis A virus cellular receptor 1
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基因ID
- 171283
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UniProt
- Q3V033
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