IL23A ELISA 试剂盒
(1 reference)-
- 抗原 See all IL23A ELISA试剂盒
- IL23A (Interleukin 23, alpha subunit p19 (IL23A))
- 抗原表位
- AA 20-196, AA 23-335
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-23
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: sf21
Immunogen sequence: (M23-S335)+(L20-A196) - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <2pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: sf21
Immunogen sequence: (M23-S335)+(L20-A196) - Featured
- Discover our best selling IL23A ELISA Kit
- Top Product
- Discover our top product IL23A ELISA Kit
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Interleukin 23, alpha subunit p19 (IL23A) ELISA Kit
IL23A 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 23, alpha subunit p19 (IL23A) ELISA KitIL23A 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 23, alpha subunit p19 (IL23A) ELISA KitIL23A 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 23, alpha subunit p19 (IL23A) ELISA KitIL23A 适用: Pig Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 23, alpha subunit p19 (IL23A) ELISA KitSmall Sample IL23A 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 23, alpha subunit p19 (IL23A) ELISA KitSmall Sample IL23A 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Secreted by activated dendritic cells (at protein level). Detected in various tissues with higher expression in polarized Th1 cells and activated macrophages. .
- 板类型
- Pre-coated
- 实验流程
- mouse IL-23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-23 has been precoated onto 96-well plates. Standards(sf21, (M23-S335)+(L20-A196)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-23 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IL-23 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatant, serum or plasma (heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-23 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 255, Standard deviation: 11.73, CV(%): 4.6
- Sample 2: n=16, Mean(pg/ml): 717, Standard deviation: 40.87, CV(%): 5.7
- Sample 3: n=16, Mean(pg/ml): 1147, Standard deviation: 68.82, CV(%): 6,
- Sample 1: n=24, Mean(pg/ml): 325, Standard deviation: 18.85, CV(%): 5.8
- Sample 2: n=24, Mean(pg/ml): 836, Standard deviation: 53.5, CV(%): 6.4
- Sample 3: n=24, Mean(pg/ml): 1327, Standard deviation: 95.5, CV(%): 7.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Therapeutic effects of Clostridium butyricum on experimental colitis induced by oxazolone in rats." in: World journal of gastroenterology, Vol. 15, Issue 15, pp. 1821-8, (2009) (PubMed).
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: "Therapeutic effects of Clostridium butyricum on experimental colitis induced by oxazolone in rats." in: World journal of gastroenterology, Vol. 15, Issue 15, pp. 1821-8, (2009) (PubMed).
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- 抗原 See all IL23A ELISA试剂盒
- IL23A (Interleukin 23, alpha subunit p19 (IL23A))
- 别名
- IL23A (IL23A 产品)
- 别名
- IL-23 ELISA Kit, IL-23A ELISA Kit, IL23P19 ELISA Kit, P19 ELISA Kit, SGRF ELISA Kit, FIL1 ELISA Kit, FIL1(ZETA) ELISA Kit, FIL1Z ELISA Kit, IL-1F7 ELISA Kit, IL-1H ELISA Kit, IL-1H4 ELISA Kit, IL-1RP1 ELISA Kit, IL-37 ELISA Kit, IL1F7 ELISA Kit, IL1H4 ELISA Kit, IL1RP1 ELISA Kit, p19 ELISA Kit, il23p19 ELISA Kit, IL23 ELISA Kit, interleukin 23 subunit alpha ELISA Kit, interleukin 37 ELISA Kit, interleukin 23, alpha subunit p19 ELISA Kit, IL23A ELISA Kit, IL37 ELISA Kit, Il23a ELISA Kit
- 背景
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Protein Function: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. .
Background: Interleukin-23 subunit alpha(IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19(the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12(stSG47812).
Synonyms: Interleukin-23 subunit alpha,IL-23 subunit alpha,IL-23-A,Interleukin-23 subunit p19,IL-23p19,Il23a,
Full Gene Name: Interleukin-23 subunit alpha
Cellular Localisation: Secreted . Secreted upon association with IL12B. - 基因ID
- 83430
- UniProt
- Q9EQ14
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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