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CCL17 ELISA 试剂盒

This 人 CCL17 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 CCL17. This ELISA Kit has been cited in1 publication.
产品编号 ABIN921088
发货至: 中国

Quick Overview for CCL17 ELISA 试剂盒 (ABIN921088)

抗原

See all CCL17 ELISA试剂盒
CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

抗原表位

AA 24-94

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2-2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • 最低检测浓度

    31.2 pg/mL

    原理

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL17/TARC

    品牌

    PicoKine™

    Analytical Method

    Quantitative

    特异性

    Expression system for standard: E.coli
    Immunogen sequence: A24-S94

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    <1pg/mL

    试剂未包括

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    免疫原

    Expression system for standard: E.coli
    Immunogen sequence: A24-S94
  • 应用备注

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    说明

    Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

    Tissue Specificity: Expressed at high levels in thymus and at low levels in the lung, colon and small intestine.

    板类型

    Pre-coated

    实验流程

    human CCL17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL17 has been precoated onto 96-well plates. Standards(E.coli, A24-S94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL17 amount of sample captured in plate.

    实验流程

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CCL17 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL17 standard solution and each sample be measured in duplicate.

    实验精密度

    • Sample 1: n=16, Mean(pg/ml): 103, Standard deviation: 6.90, CV(%): 6.7
    • Sample 2: n=16, Mean(pg/ml): 421, Standard deviation: 24.84, CV(%): 5.9
    • Sample 3: n=16, Mean(pg/ml): 1214, Standard deviation: 63.13, CV(%): 5.2,
    • Sample 1: n=24, Mean(pg/ml): 110, Standard deviation: 10.67, CV(%): 9.7
    • Sample 2: n=24, Mean(pg/ml): 462, Standard deviation: 42.97, CV(%): 9.3
    • Sample 3: n=24, Mean(pg/ml): 1283, Standard deviation: 110.34, CV(%): 8.6

    限制

    仅限研究用
  • 注意事项

    Avoid multiple freeze-thaw cycles.

    储存条件

    -20 °C,4 °C

    储存方法

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    有效期

    12 months
  • Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." in: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, (2016) (PubMed).

  • 抗原 See all CCL17 ELISA试剂盒

    CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

    别名

    CCL17

    背景

    Protein Function: Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. .

    Background: Chemokine(C-C motif) ligand 17(CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine(TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.

    Synonyms: C-C motif chemokine 17,CC chemokine TARC,Small-inducible cytokine A17,Thymus and activation-regulated chemokine,CCL17,SCYA17, TARC,

    Full Gene Name: C-C motif chemokine 17

    Cellular Localisation: Secreted.

    基因ID

    6361

    UniProt

    Q92583
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