CST3 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for CST3 ELISA 试剂盒 (ABIN921087)
抗原
See all CST3 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 312 pg/mL
-
原理
- Sandwich Mouse Cystatin C/CST3 ELISA Kit to quantitate Mouse Cst3 in cell culture supernatants, serum, plasma ( heparin, EDTA) and urine.
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NS0
Immunogen sequence: M1-A140Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Cystatin C (CST3) ELISA Kit
CST3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 小鼠 Colorimetric Sandwich ELISA 2.5-160 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 人 Colorimetric Sandwich ELISA 7.8-500 ng/mL Urine
Cystatin C (CST3) ELISA KitCST3 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: Pig Colorimetric Sandwich ELISA 125 ng/mL - 8000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Urine
Cystatin C (CST3) ELISA KitCST3 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Cystatin C (CST3) ELISA KitCST3 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
-
实验精密度
-
Intra-Assay Precision (CV%): 6.4%, 4.2%, 7.2%
Inter-Assay Precision (CV%): 7.5%, 4.7%, 8.3% -
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
-
有效期
- 12 months
-
-
- CST3 (Cystatin C (CST3))
-
别名
- CST3
-
背景
-
Background: Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.
Gene Full Name: cystatin C
-
基因ID
- 13010
-
UniProt
- P21460
抗原 See all CST3 ELISA试剂盒
-
