CST3 ELISA 试剂盒
Quick Overview for CST3 ELISA 试剂盒 (ABIN921087)
抗原
See all CST3 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cystatin C
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: M1-A140 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: M1-A140
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Cystatin C (CST3) ELISA Kit
CST3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 小鼠 Colorimetric Sandwich ELISA 2.5-160 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 人 Colorimetric Sandwich ELISA 7.8-500 ng/mL Urine
Cystatin C (CST3) ELISA KitCST3 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Cystatin C (CST3) ELISA KitCST3 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Urine
Cystatin C (CST3) ELISA KitCST3 适用: Pig Colorimetric Sandwich ELISA 125 ng/mL - 8000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cystatin C (CST3) ELISA KitCST3 适用: 兔 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse Cystatin C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cystatin C has been precoated onto 96-well plates. Standards(NSO, M1-A140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cystatin C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cystatin C amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse Cystatin C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma( heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Cystatin C standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 3.05, Standard deviation: 0.131, CV(%): 4.3
- Sample 2: n=16, Mean(ng/ml): 6.26, Standard deviation: 0.194, CV(%): 3.1
- Sample 3: n=16, Mean(ng/ml): 13.16, Standard deviation: 0.474, CV(%): 3.6,
- Sample 1: n=24, Mean(ng/ml): 3.12, Standard deviation: 0.212, CV(%): 6.8
- Sample 2: n=24, Mean(ng/ml): 6.37, Standard deviation: 0.376, CV(%): 5.9
- Sample 3: n=24, Mean(ng/ml): 13.66, Standard deviation: 0.861, CV(%): 6.3
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CST3 (Cystatin C (CST3))
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别名
- CST3
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背景
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Protein Function: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Background: Cystatin C or cystatin 3(formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid(CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight(approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus(cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases(enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases(it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.
Synonyms: Cystatin-C,Cystatin-3,Cst3,
Full Gene Name: Cystatin-C
Cellular Localisation: Secreted. -
基因ID
- 13010
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UniProt
- P21460
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