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CCL5 ELISA 试剂盒

Name: CCL5 ELISA 试剂盒
SKU: ABIN921072
Availability: OutOfStock

This 小鼠 CCL5 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 CCL5. This ELISA Kit has been cited in 3+ publications.

ELISA

1 image

(3 references)

产品编号 ABIN921072

发货至: 中国

Datasheet as PDF

Quick Overview for CCL5 ELISA 试剂盒 (ABIN921072)

抗原

See all CCL5 ELISA试剂盒

CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

抗原表位

AA 24-91

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Discover our top product CCL5 ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Rantes

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: S24-S91

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<1pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: S24-S91
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Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit
CCL5 适用: 大鼠 AA 24-91 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit
CCL5 适用: 人 AA 24-91 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Tissue Specificity: T-cell and macrophage specific.

板类型

Pre-coated

实验流程

mouse Rantes ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Rantes has been precoated onto 96-well plates. Standards(E.coli, S24-S91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rantes is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Rantes amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse Rantes standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Rantes standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 127, Standard deviation: 6.73, CV(%): 5.3
- Sample 2: n=16, Mean(pg/ml): 285, Standard deviation: 18.24, CV(%): 6.4
- Sample 3: n=16, Mean(pg/ml): 498, Standard deviation: 35.9, CV(%): 7.2,
- Sample 1: n=24, Mean(pg/ml): 146, Standard deviation: 12.12, CV(%): 8.3
- Sample 2: n=24, Mean(pg/ml): 312, Standard deviation: 21.22, CV(%): 6.8
- Sample 3: n=24, Mean(pg/ml): 587, Standard deviation: 47.55, CV(%): 8.1
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Meng, Zhang, Wu, Wu, Li, Yan, Kopec-Harding, Wu: "In vitro modeling of COPD inflammation and limitation of p38 inhibitor - SB203580." in: International journal of chronic obstructive pulmonary disease, Vol. 11, pp. 909-17, (2017) (PubMed).

Zhang, Zheng, Liu, Shu, Mao, Bai, Hu, Luo, Ma, Cui, Wang: "Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway." in: Journal of neuroinflammation, Vol. 13, Issue 1, pp. 209, (2017) (PubMed).

Liu, An, Liu, Li, Tang, Chen: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." in: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).
抗原 See all CCL5 ELISA试剂盒

CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

别名

CCL5

背景

Protein Function: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells. .
Background: Rantes(regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
Synonyms: C-C motif chemokine 5,MuRantes,SIS-delta,Small-inducible cytokine A5,T-cell-specific protein RANTES,Ccl5,Scya5,
Full Gene Name: C-C motif chemokine 5
Cellular Localisation: Secreted.

基因ID

20304

UniProt

P30882

途径

Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration