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EPO ELISA 试剂盒

Name: EPO ELISA 试剂盒
SKU: ABIN921054
Availability: OutOfStock

This 小鼠 EPO ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 EPO.

ELISA

1 image

产品编号 ABIN921054

发货至: 中国

Quick Overview for EPO ELISA 试剂盒 (ABIN921054)

抗原

See all EPO ELISA试剂盒

EPO (Erythropoietin (EPO))

抗原表位

AA 27-192

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

46.9-3000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate

Discover our top product EPO ELISA Kit

最低检测浓度

46.9 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse EPO

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: A27-R192

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<15pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: A27-R192
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应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Tissue Specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

板类型

Pre-coated

实验流程

mouse EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EPO has been precoated onto 96-well plates. Standards(NSO, A27-R192)and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse EPO amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin) or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse EPO standard solution and each sample is measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 225, Standard deviation: 10.13, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 457, Standard deviation: 17.4, CV(%): 3.8
- Sample 3: n=16, Mean(pg/ml): 1046, Standard deviation: 55.4, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): 246, Standard deviation: 23.12, CV(%): 9.4
- Sample 2: n=24, Mean(pg/ml): 486, Standard deviation: 36.45, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 1118, Standard deviation: 43.6, CV(%): 3.9
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
抗原 See all EPO ELISA试剂盒

EPO (Erythropoietin (EPO))

别名

EPO

物质类

Hormone

背景

Protein Function: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.
Synonyms: Erythropoietin,Epo,
Full Gene Name: Erythropoietin
Cellular Localisation: Secreted.

基因ID

13856

UniProt

P07321

途径

JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity