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ANG ELISA 试剂盒

Name: ANG ELISA 试剂盒
SKU: ABIN921052
Availability: OutOfStock

This 人 ANG ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 ANG. This ELISA Kit has been cited in 4+ publications.

ELISA

1 image

(4 references)

产品编号 ABIN921052

发货至: 中国

Quick Overview for ANG ELISA 试剂盒 (ABIN921052)

抗原

See all ANG ELISA试剂盒

ANG (Angiogenin (ANG))

抗原表位

AA 25-147

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

78-5000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Discover our top product ANG ELISA Kit

最低检测浓度

78 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ANG

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: Q25-P147

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<12pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: Q25-P147
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ANG 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Belongs to the pancreatic ribonuclease family.
Tissue Specificity: Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. .

板类型

Pre-coated

实验流程

human ANG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANG has been precoated onto 96-well plates. Standards(E.coli, Q25-P147) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANG amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human ANG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ANG standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 312, Standard deviation: 9.4, CV(%): 3
- Sample 2: n=16, Mean(pg/ml): 672, Standard deviation: 23.52, CV(%): 3.5
- Sample 3: n=16, Mean(pg/ml): 1663, Standard deviation: 54.9, CV(%): 3.3,
- Sample 1: n=24, Mean(pg/ml): 635, Standard deviation: 45.72, CV(%): 7.2
- Sample 2: n=24, Mean(pg/ml): 1856, Standard deviation: 165.2, CV(%): 8.9
- Sample 3: n=24, Mean(pg/ml): 3489, Standard deviation: 300, CV(%): 8.6
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Shi, Zhang, Fu, Lu, Ye, Xiao: "Angiotensin II as a morphogenic cytokine stimulating fibrogenesis of human tenon's capsule fibroblasts." in: Investigative ophthalmology & visual science, Vol. 56, Issue 2, pp. 855-64, (2015) (PubMed).

Bai, Li, Tian, Zhou: "Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes." in: PLoS ONE, Vol. 9, Issue 4, pp. e96117, (2014) (PubMed).

Li, Fan, Song, Zhang, Chen, Li, Mi, Ma, Song, Tao, Li: "Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma." in: PLoS ONE, Vol. 8, Issue 9, pp. e75388, (2013) (PubMed).

Jerosch, Castro, Colemont: "An isolated reactive synovitis of the sternoclavicular joint." in: Acta orthopaedica Belgica, Vol. 55, Issue 2, pp. 222-4, (1989) (PubMed).
抗原 See all ANG ELISA试剂盒

ANG (Angiogenin (ANG))

别名

ANG

背景

Protein Function: Binds to actin on the surface of endothelial cells, once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. .
Background: Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
Synonyms: Angiogenin,3.1.27.-,Ribonuclease 5,RNase 5,ANG,RNASE5,
Full Gene Name: Angiogenin
Cellular Localisation: Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.

基因ID

283

UniProt

P03950