Motilin ELISA 试剂盒
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Quick Overview for Motilin ELISA 试剂盒 (ABIN8126684)
抗原
See all Motilin (MLN) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 93.75 pg/mL
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原理
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
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Analytical Method
- Quantitative
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特异性
- This kit recognizes Rat MTL in samples.No significant cross-reactivity or interference between Rat MTL and analogues was observed
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灵敏度
- 56.25 pg/mL
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组件
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
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Motilin (MLN) ELISA Kit
MLN 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Motilin (MLN) ELISA KitMLN 适用: 人 Colorimetric Competition ELISA 74.07 pg/mL - 6000 pg/mL Plasma, Serum
Motilin (MLN) ELISA KitMLN 适用: 犬 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Motilin (MLN) ELISA KitMLN 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Motilin (MLN) ELISA KitMLN 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Motilin (MLN) ELISA KitMLN 适用: 小鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Motilin (MLN) ELISA KitMLN 适用: 大鼠 Colorimetric Competition ELISA 74.07 pg/mL - 6000 pg/mL Plasma, Serum
Motilin (MLN) ELISA KitMLN 适用: 人 Colorimetric Competition ELISA 15.63 pg/mL - 1000 pg/mL Plasma, Serum
Motilin (MLN) ELISA KitMLN 适用: 人 Colorimetric Competition ELISA 15.63 pg/mL - 1000 pg/mL Plasma, Serum
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样本量
- 50 μL
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实验时间
- 3.5 h
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板类型
- Pre-coated
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实验流程
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- Add 50 µL standard or sample to each well. Immediately add 50 µL Biotinylated Detection Antibody to each well. Incubate for 45 min at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate to each well. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
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试剂准备
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- Equilibrate all reagents to room temperature (18-25 °C) before use. If the kit will not be used up in one assay, please only take out the necessary strips and reagents for present experiment, and store the remaining strips and reagents at required condition.
- Centrifuge the standard at 10,000x g for 1 min. Reconstitute with 1 mL of the Standard & Sample Diluent, allow to stand for 10 minutes, and gently invert several times until completely dissolved. Alternatively, vortex briefly at low speed, remove bubbles by low-speed centrifugation if necessary.This produces a 6000 pg/mL stock solution. Prepare serial dilutions according to experimental requirements. Recommended dilution gradient: 6000, 3000, 1500, 750, 375, 187.5, 93.75, 0 pg/mL Serial Dilution Procedure: Dispense 500 μL of Standard & Sample Diluent into 7 EP tubes, respectively. Transfer 500 μL of the stock solution into the first tube. Sequentially transfer 500 μL from the preceding tube into the next tube for subsequent dilutions. Designate the last tube as the blank, do not transfer any solution into it. Aliquot the stock and store at -20 ° C. Use within two weeks and avoid repeated freeze-thaw cycles. Serially diluted standards should be prepared immediately, prior to use.
- Calculate the required volume (50 μL/well) plus a slight excess. Centrifuge the Concentrated Biotinylated Detection Antibody (100x) at 800 x g for 1 minute. Dilute to 1x with Biotinylated Detection Ab Diluent at a ratio of 1:99. Prepare the working solution immediately, prior to use.
- HRP Conjugate Working Solution: Calculate the required volume (100 μL/well) plus a slight excess. Centrifuge the Concentrated HRP Conjugate (100x) at 800 x g for 1 minute. Centrifuge the Concentrated HRP Conjugate (100x) at 800 x g for 1 minute. Prepare the working solution immediately, prior to use.
- Wash Buffer: Prepare the working Wash Buffer by diluting 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water yielding 750 mL. Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved. For same day use only.
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样品制备
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- It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
- If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
- If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
- Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.
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实验精密度
- Both intra-CV and inter-CV are < 10%.
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
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- For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The reference standard, biotinylated detection antibody, HRP conjugate, and 96-well strip plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
- For used kits: When the kit is used, the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
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有效期
- 12 months
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- Motilin (MLN)
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别名
- Motilin
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背景
- Target Synonym: MLN
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UniProt
- A8IRI0
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途径
- Hormone Activity
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