IL-21 ELISA 试剂盒
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Quick Overview for IL-21 ELISA 试剂盒 (ABIN8126247)
抗原
See all IL-21 (IL21) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.25 pg/mL
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原理
- This assay employs an antibody specific for Anti-Mouse IL-21 coated on a 96-well plate.
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Analytical Method
- Quantitative
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-21 concentrations in serum, plasma. Detection Range: 31.25 - 2000 pg/mL Sensitivity: <18.75pg / ml
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板类型
- Pre-coated
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实验流程
- This assay employs an antibody specific for Anti-Mouse IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound.The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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限制
- 仅限研究用
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储存方法
- Please refer to the Manual
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- IL-21 (IL21) (Interleukin 21 (IL21))
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别名
- IL-21 (Interleukin 21)
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原 See all IL-21 (IL21) ELISA试剂盒
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