IL-13 ELISA 试剂盒
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Quick Overview for IL-13 ELISA 试剂盒 (ABIN8126245)
抗原
See all IL-13 (IL13) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.25 pg/mL
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原理
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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灵敏度
- 18.75 pg/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-13 concentrations in serum, plasma. Detection Range: 31.25 - 2000 pg/mL Sensitivity: < 18.75 pg/mL
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板类型
- Pre-coated
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实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-13 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader, and then the concentration of IL-13 can be calculated.
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限制
- 仅限研究用
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储存方法
- Please refer to the Manual
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- IL-13 (IL13) (Interleukin 13 (IL13))
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别名
- IL-13 (Interleukin 13)
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途径
- JAK/STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
抗原 See all IL-13 (IL13) ELISA试剂盒
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