Osteopontin ELISA 试剂盒
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Quick Overview for Osteopontin ELISA 试剂盒 (ABIN8126238)
抗原
See all Osteopontin (SPP1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This assay employs an antibody specific for Anti-human OPN coated on a 96-well plate.
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Analytical Method
- Quantitative
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灵敏度
- 93.75 pg/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Human OPN concentrations in serum, plasma. Detection Range: 0.156 - 10 ng/mL Sensitivity: < 93.75 pg/mL
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板类型
- Pre-coated
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实验流程
- This assay employs an antibody specific for Anti-human OPN coated on a 96-well plate. Standards and samples are pipetted into the wells and OPN present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human OPN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of OPN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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限制
- 仅限研究用
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储存方法
- Please refer to the Manual
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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别名
- OPN (Osteopontin)
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途径
- Regulation of Cell Size
抗原 See all Osteopontin (SPP1) ELISA试剂盒
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