GM-CSF ELISA 试剂盒
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Quick Overview for GM-CSF ELISA 试剂盒 (ABIN8126236)
抗原
See all GM-CSF (CSF2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.9 pg/mL
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原理
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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灵敏度
- 2.34 pg/mL
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产品特性
- This immunoassay kit allows for the in vitro quantitative determination of Human GMCSF concentrations in serum, plasma. Detection Range: 3.9 - 250 pg/mL Sensitivity: < 2.34 pg/mL
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) ELISA KitCSF2 适用: 大鼠 AA 1-127 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
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板类型
- Pre-coated
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实验流程
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human GMCSF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human GMCSF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GMCSF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human GMCSF can be calculated using the standard curve.
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限制
- 仅限研究用
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储存方法
- Please refer to the Manual
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- GMCSF (Granulocyte Macrophage Colony Stimulating Factor)
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原 See all GM-CSF (CSF2) ELISA试剂盒
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