Adenovirus IgG ELISA 试剂盒
人 Adenovirus IgG ELISA Kit, Colorimetric is an assay for quantification of 人 Adenovirus IgG.
产品编号 ABIN8092270
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Adenovirus IgG ELISA 试剂盒 (ABIN8092270)
抗原
See all Adenovirus IgG (HAdV IgG) products
Adenovirus IgG (HAdV IgG)
(Anti-Human Adenovirus IgG (HAdV IgG))
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
原理
- Human ADV-IgG (adenovirus-Immunoglobulin G) ELISA Kit
-
Analytical Method
- Qualitative
-
特异性
- Specifically binds with ADV-IgG , no obvious cross reaction with other analogues.
-
试剂未包括
-
- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
Indirect ELISA
-
样本量
- 100 μL
-
实验时间
- 3 h
-
板类型
- Pre-coated
-
实验流程
- Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-antibody working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid direct light exposure to antibody solutions
-
储存条件
- 4 °C
-
储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
-
有效期
- 12 months
-
-
- Adenovirus IgG (HAdV IgG) (Anti-Human Adenovirus IgG (HAdV IgG))
-
别名
- ADV-IgG
-
背景
- ADV IgG, adenovirus-Immunoglobulin G
抗原 See all Adenovirus IgG (HAdV IgG) products
-
You are here:
