NPY ELISA 试剂盒
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Quick Overview for NPY ELISA 试剂盒 (ABIN8091125)
抗原
See all NPY ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.25 pg/mL
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原理
- Chicken NPY (Neuropeptide Y) ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with NPY , no obvious cross reaction with other analogues.
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灵敏度
- 18.75 pg/mL
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组件
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- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Biotin-labeled Antibody
- HRP-Streptavidin Conjugate(SABC
- 100X)
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- Microorifice oscillator
- or shake bed 37 °C incubator (When using a water bath incubator
- ensure the internal air temperature is 35-38 °C, When using the cell CO2 incubator
- use a sealed bag to isolate the microplate)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Neuropeptide Y (NPY) ELISA Kit
NPY 适用: 人 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 大鼠 Colorimetric Competition ELISA 2.47 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 人 Colorimetric Competition ELISA 4.94 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 小鼠 Colorimetric Competition ELISA 2.47 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: Cow Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Neuropeptide Y (NPY) ELISA KitNPY 适用: 小鼠 Colorimetric Sandwich ELISA 1.87 pg/mL - 120 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 人 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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样本量
- 100 μL
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实验时间
- 4 h
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板类型
- Pre-coated
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实验流程
- Step 1: Add 100 µL standard or sample into each well, Seal the plate and incubate on a microorifice oscillator or shake bed at 200 rpm and 2-8 °C for 2 hours with shaking, ensuring no spillage from any well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Washing: Wash the plate twice without immersion. Step 2: Add 100 µL biotin-labeled antibody working solution into each well, Seal the plate and incubate on a microorifice oscillator or shake bed at 200 rpm and room temperature (25 °C ± 3 °C) for 1 hours with shaking, ensuring no spillage from any well. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL SABC working solution into each well, Seal the plate and incubate on a microorifice oscillator or shake bed at 200 rpm and room temperature (25 °C ± 3 °C) for 0.5 hours with shaking, ensuring no spillage from any well. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and static incubate for 30 minutes at room temperature (25 °C ± 3 °C). (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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- NPY (Neuropeptide Y (NPY))
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别名
- NPY
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背景
- Neuropeptide Y, Neuropeptide tyrosine, NPY, NPY
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UniProt
- P28673
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途径
- Feeding Behaviour
抗原 See all NPY ELISA试剂盒
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