UBE2I ELISA 试剂盒
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Quick Overview for UBE2I ELISA 试剂盒 (ABIN8088740)
抗原
See all UBE2I ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- Human Ubiquitin Conjugating Enzyme E2I (UBE2I) ELISA Kit for detecting UBE2I in tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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灵敏度
- 0.057 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Ubiquitin-Conjugating Enzyme E2I (UBE2I) ELISA Kit
UBE2I 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ubiquitin-Conjugating Enzyme E2I (UBE2I) ELISA KitUBE2I 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Ubiquitin-Conjugating Enzyme E2I (UBE2I) ELISA KitUBE2I 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Ubiquitin-Conjugating Enzyme E2I (UBE2I) ELISA KitUBE2I 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Samples, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验精密度
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C for one month, Long term: see manual.
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有效期
- 12 months
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- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
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别名
- Ubiquitin Conjugating Enzyme E2I
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背景
- Gene Aliases: P18, UBC9, UBCE9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-protein ligase I, SUMO-conjugating enzyme UBC9
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UniProt
- P63279
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
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