SMPD3 ELISA 试剂盒
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Quick Overview for SMPD3 ELISA 试剂盒 (ABIN8087307)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.312 ng/mL
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原理
- Rat Sphingomyelin Phosphodiesterase 3 (SMPD3) ELISA Kit for detecting SMPD3 in tissue homogenates, cell lysates
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Analytical Method
- Quantitative
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灵敏度
- 0.117 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Sphingomyelin Phosphodiesterase 3 (SMPD3) ELISA Kit
SMPD3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Sphingomyelin Phosphodiesterase 3 (SMPD3) ELISA KitSMPD3 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Sphingomyelin Phosphodiesterase 3 (SMPD3) ELISA KitSMPD3 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验精密度
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C for one month, Long term: see manual.
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有效期
- 12 months
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- SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))
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别名
- Sphingomyelin Phosphodiesterase 3
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背景
- Gene Aliases: NSMASE2, Neutral Sphingomyelinase II
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基因ID
- 94338
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UniProt
- D4A8L3
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途径
- Hormone Transport
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