PKC epsilon CLIA Kit
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北京 101111
Quick Overview for PKC epsilon CLIA Kit (ABIN8085863)
抗原
See all PKC epsilon (PRKCE) CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.14 ng/mL
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原理
- Human Protein Kinase C Epsilon (PKCe) CLIA Kit for detecting PKCe in tissue homogenates
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Analytical Method
- Quantitative
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灵敏度
- 0.07 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验精密度
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C for one month, Long term: see manual.
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有效期
- 12 months
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- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
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别名
- Protein Kinase C Epsilon
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背景
- Gene Aliases: PKC-E, PRKCE, PRKC-E, NPKC-Epsilon
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基因ID
- 5581
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UniProt
- Q02156
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途径
- TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
抗原 See all PKC epsilon (PRKCE) CLIA Kits
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