Leptin CLIA Kit
Our Local Distributor
北京 101111
Quick Overview for Leptin CLIA Kit (ABIN8082793)
抗原
See all Leptin (LEP) CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 27.4 pg/mL
-
原理
- Rat Leptin (LEP) CLIA Kit for detecting LEP in serum, plasma, tissue homogenates, cell culture supernates
-
Analytical Method
- Quantitative
-
灵敏度
- 12.2 pg/mL
-
组件
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
试剂未包括
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
Leptin (LEP) ELISA Kit
LEP 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: Salmon Colorimetric Competition ELISA 62.5 pg/mL - 1000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP 适用: 兔 Colorimetric Competition ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 兔 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 小鸡 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
样本量
- 100 μL
-
实验时间
- 3 h
-
板类型
- Pre-coated
-
实验精密度
-
Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- 4°C for one month, Long term: see manual.
-
有效期
- 12 months
-
-
- Leptin (LEP)
-
别名
- Leptin
-
背景
- Gene Aliases: OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein
-
基因ID
- 25608
-
UniProt
- P50596
-
途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
抗原 See all Leptin (LEP) CLIA Kits
-
