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IL12A ELISA 试剂盒

小鼠 IL12A ELISA Kit, Colorimetric is an assay for quantification of 小鼠 IL12A.
产品编号 ABIN8081767
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IL12A ELISA 试剂盒 (ABIN8081767)

抗原

See all IL12A ELISA试剂盒
IL12A (Interleukin 12 alpha (IL12A))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
  • 最低检测浓度

    15.6 pg/mL

    原理

    Mouse Interleukin 12A (IL12A) ELISA Kit for detecting IL12A in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

    Analytical Method

    Quantitative

    灵敏度

    5.7 pg/mL

    组件

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    试剂未包括

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    样本量

    100 μL

    实验时间

    3 h

    板类型

    Pre-coated

    实验精密度

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    4°C for one month, Long term: see manual.

    有效期

    12 months
  • 抗原 See all IL12A ELISA试剂盒

    IL12A (Interleukin 12 alpha (IL12A))

    别名

    Interleukin 12A

    背景

    Gene Aliases: p35, IL12-A, CLMF1, NFSK, NKSF1, Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, NF Cell Stimulatory Factor Chain 1

    基因ID

    16159

    UniProt

    P43431

    途径

    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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