TNK1 ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 TNK1.
产品编号 ABIN8071212
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for TNK1 ELISA 试剂盒 (ABIN8071212)
抗原
See all TNK1 ELISA试剂盒
TNK1
(Tyrosine Kinase, Non-Receptor, 1 (TNK1))
抗原表位
pan, pTyr277
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Lysate, Tissue Lysate
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原理
- Phospho-TNK1 (Tyr277) ELISA Kit measures phosphorylated TNK1 (Tyr277) and total (pan) TNK1 in cell and tissue lysates.
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Analytical Method
- Semi-Quantitative
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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- TNK1 (Tyrosine Kinase, Non-Receptor, 1 (TNK1))
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别名
- TNK1
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背景
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Gene Symbols: TNK1
Protein Name (Synonyms): Non-receptor tyrosine-protein kinase TNK1 (EC 2.7.10.2) (CD38 negative kinase 1)
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基因ID
- 8711
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UniProt
- Q13470
抗原 See all TNK1 ELISA试剂盒
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