Transglutaminase 2 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for Transglutaminase 2 ELISA 试剂盒 (ABIN8071128)
抗原
See all Transglutaminase 2 (TGM2) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- Tissue Transglutaminase 2 (TGM2) Human IgG ELISA Kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgG autoantibody against TGM2 in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.
-
Analytical Method
- Quantitative
-
组件
-
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- Sample Diluent
-
试剂未包括
-
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
-
-
Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2) ELISA Kit
TGM2 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2) ELISA KitTGM2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2) ELISA KitTGM2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2) ELISA KitHigh Sensitivity TGM2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2) ELISA KitSmall Sample TGM2 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2) ELISA KitTGM2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2) ELISA KitTGM2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 mU/mL - 10 mU/mL Plasma, Serum, Tissue Homogenate
Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2) ELISA KitSmall Sample TGM2 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
-
- Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))
-
别名
- Transglutaminase 2
-
背景
-
Gene Symbols: TGM2
Protein Name (Synonyms): Protein-glutamine gamma-glutamyltransferase 2 (EC 2.3.2.13) (Erythrocyte transglutaminase) (Heart G alpha(h)) (hhG alpha(h)) (Isopeptidase TGM2) (EC 3.4.-.-) (Protein G alpha(h)) (G(h)) (Protein-glutamine deamidase TGM2) (EC 3.5.1.44) (Protein-glutamine dopaminyltransferase TGM2) (EC 2.3.1.-) (Protein-glutamine histaminyltransferase TGM2) (EC 2.3.1.-) (Protein-glutamine noradrenalinyltransferase TGM2) (EC 2.3.1.-) (Protein-glutamine serotonyltransferase TGM2) (EC 2.3.1.-) (Tissue transglutaminase) (tTG) (tTgase) (Transglutaminase C) (TG(C)) (TGC) (TGase C) (Transglutaminase H) (TGase H) (Transglutaminase II) (TGase II) (Transglutaminase-2) (TG2) (TGase-2) (hTG2)
-
基因ID
- 7052
-
UniProt
- P21980
-
途径
- Tube Formation, Thromboxane A2 Receptor Signaling
抗原 See all Transglutaminase 2 (TGM2) ELISA试剂盒
-
