TGFB3 ELISA 试剂盒
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Quick Overview for TGFB3 ELISA 试剂盒 (ABIN8071120)
抗原
See all TGFB3 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 4.3 pg/mL
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原理
- Human TGF beta 3 ELISA Kit for Cell Culture Supernatants and Serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Amphiregulin, BDNF, bFGF, BMP-4, BMP-5, BMP-7, betaNGF, EGF, EGFR, EG-VEGF (PK1), FGF-4, FGF-7 (KGF), GDF-15, GDNF, Growth Hormone, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, Insulin, MCSF R, NGFR (TNFRSF16), NT-3, NT-4, Osteoprotegerin (TNFRSF11B), PDGF-AA, PLGF, SCF, SCF R (CD117/c-kit), TGF alpha, TGF beta 1, VEGF-A, VEGFR2, VEGFR3, VEGF-D.
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Transforming Growth Factor, beta 3 (TGFB3) ELISA Kit
TGFB3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 兔 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 马 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitSmall Sample TGFB3 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 马 AA 346-457 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Urine
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应用备注
- Suggested Plasma Dilution: 2 fold after treatment
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))
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别名
- TGF beta 3
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背景
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Gene Symbols: TGFB3
Protein Name (Synonyms): Transforming growth factor beta-3 proprotein [Cleaved into: Latency-associated peptide (LA
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UniProt
- P10600
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途径
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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