TXN ELISA 试剂盒
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Quick Overview for TXN ELISA 试剂盒 (ABIN8071011)
抗原
See all TXN ELISA试剂盒抗原表位
适用
检测方法
实验类型
应用范围
样品类型
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原理
- Measures nitrated Thioredoxin-1 (TXNDC2) and total Thioredoxin-1 in human cell and tissue lysates, enabling precise assessment of Thioredoxin-1 nitration by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.
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Analytical Method
- Quantitative
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组件
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- Size / Qty
- Microplate Coated With Anti-Pan-Thioredoxin-1 Antibody
- Anti-Nitration Detection Antibody
- Anti-Thioredoxin-1 Detection Antibody
- HRP-Conjugated Streptavidin
- 200 μL
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare sample dilutions
- Distilled or deionized water
- Shaker
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Thioredoxin (TXN) ELISA Kit
TXN 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thioredoxin (TXN) ELISA KitTXN 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Thioredoxin (TXN) ELISA KitTXN 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Thioredoxin (TXN) ELISA KitTXN 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Thioredoxin (TXN) ELISA KitTXN 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Thioredoxin (TXN) ELISA KitTXN 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thioredoxin (TXN) ELISA KitTXN 适用: 人 AA 2-105 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Thioredoxin (TXN) ELISA KitTXN 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Thioredoxin (TXN) ELISA KitTXN 适用: 犬 Colorimetric Competition ELISA 250-5000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed.
- Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- TXN (Thioredoxin (TXN))
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别名
- Thioredoxin-1
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背景
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Gene Symbols: TXN, TRDX, TRX, TRX1
Background: The Nitrated-Thioredoxin-1 and Total-Thioredoxin-1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human nitrated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan Thioredoxin-1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Thioredoxin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-nitration antibody is used to detect nitrated Thioredoxin-1 or biotinylated anti-Thioredoxin-1 antibody is used to detect total Thioredoxin-1 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of nitrated Thioredoxin-1 or total Thioredoxin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Thioredoxin (Trx), ATL-derived factor (ADF), Surface-associated sulphydryl protein (SASP)
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基因ID
- 7295
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UniProt
- P10599
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途径
- Carbohydrate Homeostasis, Cell RedoxHomeostasis
抗原 See all TXN ELISA试剂盒
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