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tau ELISA 试剂盒

Name: tau ELISA 试剂盒
SKU: ABIN8070974
Availability: OutOfStock

人, 小鼠和大鼠 tau ELISA Kit, Colorimetric is an assay for quantification of 人, 小鼠和大鼠 tau.

产品编号 ABIN8070974

发货至: 中国

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Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for tau ELISA 试剂盒 (ABIN8070974)

抗原

See all tau ELISA试剂盒

tau (tau Protein)

抗原表位

pSer202, pThr205

适用

人, 小鼠, 大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

2.7 pM - 1961 pM

应用范围

ELISA

样品类型

Cerebrospinal Fluid, Plasma, Serum

Discover our top product tau ELISA Kit

最低检测浓度

2.7 pM

原理

Phospho-Tau (Ser202/Thr205) ELISA is a phosphorylation assay kit for the quantitative measurement of phosphorylated Tau at Ser202/Thr205 in Human, Mouse and Rat serum, plasma and CSF.

Analytical Method

Quantitative

特异性

This ELISA antibody pair detects human, mouse and rat Phospho Tau (S202/T205).

组件
- Pan Tau Microplate: 96 wells (12 strips x 8 wells) coated with anti-pan- Tau.
- Wash Buffer
- Phospho Tau Standard Protein
- Phospho Detection Antibody Tau (Ser202/Thr205)
- HRP-Streptavidin concentrate
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Positive Control Sample
,
- Pan Tau Microplate: 96 wells (12 strips x 8 wells) coated with anti-pan- Tau.
- Wash Buffer
- Phospho Tau Standard Protein
- Phospho Detection Antibody Tau (Ser202/Thr205)
- HRP-Streptavidin concentrate
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Positive Control Sample
试剂未包括
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
tau Protein ELISA Kit
适用: 大鼠 phosphorylated Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

tau Protein ELISA Kit
适用: 人 phosphorylated Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate

tau Protein ELISA Kit
High Sensitivity 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

tau Protein ELISA Kit
High Sensitivity 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

tau Protein ELISA Kit
High Sensitivity 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate

tau Protein ELISA Kit
适用: 大鼠 phosphorylated Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

tau Protein ELISA Kit
Small Sample 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

tau Protein ELISA Kit
Small Sample 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

tau Protein ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 100 pg/mL - 25000 pg/mL Cell Culture Supernatant, Plasma, Serum

tau Protein ELISA Kit
适用: 人, 小鼠, 大鼠 pThr231 Colorimetric Cell ELISA Cell Culture Cells

应用备注

Suggested Plasma Dilution: 5-fold to 50- fold dilution

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL of each standard and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles. For prepared reagent storage, see table in manual.
抗原 See all tau ELISA试剂盒

tau (tau Protein)

别名

Tau

背景

Gene Symbols: MAPT,MAPTL,MTBT1,TAU
Background: Phospho-Tau (Ser202/Thr205) Quantitative ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in Human, Mouse & Rat serum, plasma and CSF. By determining phosphorylated Tau protein in your experimental model system, you can verify pathway activation in your sample. You can simultaneously measure numerous different samples without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human, Mouse & Rat phospho-Tau. An anti-pan Tau antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and Tau present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phospho-Tau (Ser202/Thr205) antibody is used to detect phosphorylated Tau. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tau (Ser202/Thr205) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

UniProt

P10636, P19332, P10637