tau ELISA 试剂盒
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北京 101111
Quick Overview for tau ELISA 试剂盒 (ABIN8070969)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.123 ng/mL
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原理
- Phospho-TAU (Ser199) Quantitative ELISA Kit is a convenient and sensitive assay to detect phosphorylated Tau at Serine-199 in human, mouse cell lysate samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human and mouse Phospho TAU (Ser199).
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组件
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- Pre-coated 96-well microplate
- Lyophilized Standard
- Anti-phospho Detection Antibody
- HRP-Conjugated Secondary Antibody
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare the positive control or sample dilutions
- Distilled or deionized water
- Shaker
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tau Protein ELISA Kit
适用: 大鼠 phosphorylated Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
tau Protein ELISA Kit适用: 人 phosphorylated Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
tau Protein ELISA KitHigh Sensitivity 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
tau Protein ELISA KitHigh Sensitivity 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
tau Protein ELISA KitHigh Sensitivity 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
tau Protein ELISA Kit适用: 大鼠 phosphorylated Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
tau Protein ELISA KitSmall Sample 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
tau Protein ELISA KitSmall Sample 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
tau Protein ELISA Kit适用: 人 Colorimetric Sandwich ELISA 100 pg/mL - 25000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and positive control as instructed.
- Add 100 μL of each standard and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-conjugated secondary antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- tau (tau Protein)
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别名
- Tau
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背景
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Gene Symbols: MAPT, MAPTL, MTBT1, TAU
Background: Phospho-TAU (Ser199) Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human, Mouse phosphorylated TAU (Ser199). An anti-pan TAU antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and TAU present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-TAU (Ser199) antibody is used to detect phosphorylated TAU. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TAU (Ser199) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau (PHF-tau)
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基因ID
- 413717762
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UniProt
- P10636, P19332, P10637
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