SNCA ELISA 试剂盒
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Quick Overview for SNCA ELISA 试剂盒 (ABIN8070957)
抗原
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适用
检测方法
实验类型
应用范围
样品类型
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原理
- Human Nitrated Alpha-synuclein (Tyr125/133) Quantitative ELISA Kit. This ELISA is for measuring Nitrated Alpha-synuclein (Tyr125/133) in human cell lysates.
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Analytical Method
- Quantitative
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特异性
- The antibody pair provided in this kit recognizes human Nitrated Alpha-synuclein (Tyr125/133).
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组件
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- Anti-pan-Alpha-synuclein Microplate
- Nitrated Alpha-synuclein Standard
- Anti- Nitrated Alpha-synuclein (Tyr125/133) Antibody
- HRP-conjugated anti-rabbit IgG concentrate
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare positive control or sample dilutions
- Distilled or deionized water
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Synuclein, alpha (SNCA) ELISA Kit
SNCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cerebrospinal Fluid, Plasma, Serum
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples, and Positive Control as instructed. in the manual.
- Add 100 μL Standard to each well.
- Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well.
- Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-conjugated anti-rabbit IgG concentrate solution.
- Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- SNCA (Synuclein, alpha (SNCA))
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别名
- Alpha-Synuclein
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背景
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Gene Symbols: SNCA NACP PARK1
Background: Nitrated Alpha-synuclein (Tyr125/133) Quantitative ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Nitrated Alpha-synuclein (Tyr125/133). An anti-pan Alpha-synuclein antibody has been coated onto a 96-well plate. Standard and samples are pipetted into the wells and Alpha-synuclein present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and rabbit anti-Nitrated Alpha-synuclein (Tyr125/133) antibody is used to detect only Nitrated protein. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Nitrated Alpha-synuclein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Alpha-synuclein (Non-A beta component of AD amyloid) (Non-A4 component of amyloid precursor) (NACP)
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基因ID
- 6622
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UniProt
- P37840
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途径
- Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity
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