SNCA ELISA 试剂盒
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Quick Overview for SNCA ELISA 试剂盒 (ABIN8070954)
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适用
检测方法
实验类型
应用范围
样品类型
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原理
- Phospho-Alpha-synuclein (S129) and Total Alpha-synuclein ELISA Kit is a convenient and sensitive assay that can monitor the activation or function of important biological pathways in Human cell lysates
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Analytical Method
- Semi-Quantitative
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特异性
- The antibody pair provided in this kit recognizes human alpha-synuclein (SNCA) phosphorylated at site Serine-129.
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组件
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- Pre-Coated 96-well Strip Microplate
- Positive Control
- Anti-Phospho Detection Antibody
- Anti-Pan Detection Antibody (*not included in phospho-only version)
- HRP-Conjugated Secondary Antibody
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Shaker
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare the positive control or sample dilutions
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Synuclein, alpha (SNCA) ELISA Kit
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Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cerebrospinal Fluid, Plasma, Serum
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Synuclein, alpha (SNCA) ELISA KitSNCA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and positive control as instructed.
- Add 100 μL positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- SNCA (Synuclein, alpha (SNCA))
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别名
- Alpha-Synuclein
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背景
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Gene Symbols: SNCA, NACP, PARK1
Protein Name (Synonyms): Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor (NACP)
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基因ID
- 6622
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UniProt
- P37840
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途径
- Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity
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