SMN1 ELISA 试剂盒
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Quick Overview for SMN1 ELISA 试剂盒 (ABIN8070924)
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See all SMN1 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- Smith (Sm) Antigen (SmA) Human IgA ELISA Kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgA autoantibody against SmA in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.
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Analytical Method
- Quantitative
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组件
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- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- Sample Diluent
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
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Survival of Motor Neuron 1, Telomeric (SMN1) ELISA Kit
SMN1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Survival of Motor Neuron 1, Telomeric (SMN1) ELISA KitSMN1 适用: 小鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- SMN1 (Survival of Motor Neuron 1, Telomeric (SMN1))
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别名
- SMA
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途径
- Ribonucleoprotein Complex Subunit Organization
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