Somatostatin ELISA 试剂盒
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北京 101111
Quick Overview for Somatostatin ELISA 试剂盒 (ABIN8070845)
抗原
See all Somatostatin (SST) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 ng/mL
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原理
- Human/Mouse/Rat Pro Somatostatin EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human, mouse, and rat pro-Somatostatin, not active Somatostatin.
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灵敏度
- 10.9 ng/mL
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Somatostatin (SST) ELISA Kit
SST 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Somatostatin (SST) ELISA KitSST 适用: 大鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Plasma, Serum
Somatostatin (SST) ELISA KitSST 适用: 小鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Plasma, Serum
Somatostatin (SST) ELISA KitSST 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Somatostatin (SST) ELISA KitSST 适用: 人 Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Somatostatin (SST) ELISA KitSST 适用: 小鼠 Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Somatostatin (SST) ELISA KitSST 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Somatostatin (SST) ELISA KitSST 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Somatostatin (SST) ELISA KitSST 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Somatostatin (SST) ELISA KitSST 适用: Pig Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Plasma, Serum
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应用备注
- Suggested Plasma Dilution: Human: 2x / Mouse: 2x / Rat: 2x
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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- Somatostatin (SST)
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别名
- Somatostatin
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物质类
- Chemical
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背景
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Gene Symbols: SST
Background: Somatostatin, also known as growth hormone-inhibiting hormone (GHIH) that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin regulates insulin and glucagon.
Somatostatin is secreted in several locations in the digestive system: stomach, intestine and delta cells of the pancreas. In the stomach, somatostatin acts on the acid-producing parietal cells via G-coupled receptor to reduce secretion. Somatostatin also indirectly decreases stomach acid production by preventing the release of other hormones including gastrin, secretin and histamine which effectively slows down the digestive process.
Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. In all vertebrates, there exist six different somatostatin genes that have been named SS1, SS2, SS3, SS4, SS5, and SS6. The six different genes along with the five different somatostatin receptors allow somatostatin to possess a large range of functions. Humans have only one somatostatin gene, SST.Protein Name (Synonyms): Somatostatin (Growth hormone release-inhibiting factor) [Cleaved into: Somatostatin-28, Somatostatin-14]
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基因ID
- 6750
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UniProt
- P61278
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