SUMO1 ELISA 试剂盒
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Quick Overview for SUMO1 ELISA 试剂盒 (ABIN8070839)
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适用
检测方法
实验类型
应用范围
样品类型
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原理
- Measures ubiquitinated SUMO1 and total SUMO1 in human cell and tissue lysates, enabling precise assessment of SUMO1 ubiquitination by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.
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Analytical Method
- Quantitative
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组件
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- Size / Qty
- Microplate Coated With Anti-Pan-SUMO1 Antibody
- Anti-Ubiquitination Detection Antibody
- Anti-SUMO1 Detection Antibody
- HRP-Conjugated Streptavidin
- 200 μL
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare sample dilutions
- Distilled or deionized water
- Shaker
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Small Ubiquitin Related Modifier Protein 1 (SUMO1) ELISA Kit
SUMO1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Small Ubiquitin Related Modifier Protein 1 (SUMO1) ELISA KitSUMO1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Small Ubiquitin Related Modifier Protein 1 (SUMO1) ELISA KitSUMO1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Small Ubiquitin Related Modifier Protein 1 (SUMO1) ELISA KitHigh Sensitivity SUMO1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Small Ubiquitin Related Modifier Protein 1 (SUMO1) ELISA KitSUMO1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed.
- Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))
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别名
- SUMO1
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背景
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Gene Symbols: SUMO1, SMT3C, SMT3H3, UBL1, OK/SW-cl.43
Background: The Ubiquitinated-SUMO1 and Total-SUMO1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human ubiquitinated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan SUMO1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SUMO1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-ubiquitination antibody is used to detect ubiquitinated SUMO1 or biotinylated anti-SUMO1 antibody is used to detect total SUMO1 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ubiquitinated SUMO1 or total SUMO1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Small ubiquitin-related modifier 1 (SUMO-1), GAP-modifying protein 1 (GMP1), SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C (Smt3C), Ubiquitin-like protein UBL1
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基因ID
- 7341
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UniProt
- P63165
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途径
- M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway
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