RUNX2 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for RUNX2 ELISA 试剂盒 (ABIN8070671)
抗原
See all RUNX2 ELISA试剂盒抗原表位
适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- Measures acetylated RUNX2 (CBFA1) and total RUNX2 in human cell and tissue lysates, enabling precise assessment of RUNX2 acetylation by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.
-
Analytical Method
- Quantitative
-
组件
-
- Size / Qty
- Microplate Coated With Anti-Pan-RUNX2 Antibody
- Anti-Acetylation Detection Antibody
- Anti-RUNX2 Detection Antibody
- HRP-Conjugated Streptavidin
- 200 μL
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
-
试剂未包括
-
- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare sample dilutions
- Distilled or deionized water
- Shaker
-
-
Runt-Related Transcription Factor 2 (RUNX2) ELISA Kit
RUNX2 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Runt-Related Transcription Factor 2 (RUNX2) ELISA KitRUNX2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Runt-Related Transcription Factor 2 (RUNX2) ELISA KitRUNX2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Runt-Related Transcription Factor 2 (RUNX2) ELISA KitRUNX2 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Runt-Related Transcription Factor 2 (RUNX2) ELISA KitRUNX2 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Runt-Related Transcription Factor 2 (RUNX2) ELISA KitRUNX2 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Runt-Related Transcription Factor 2 (RUNX2) ELISA KitRUNX2 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Runt-Related Transcription Factor 2 (RUNX2) ELISA KitRUNX2 适用: 兔 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Runt-Related Transcription Factor 2 (RUNX2) ELISA KitRUNX2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Runt-Related Transcription Factor 2 (RUNX2) ELISA KitRUNX2 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents and samples as instructed.
- Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
-
-
- RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))
-
别名
- RUNX2/CBFA1
-
背景
-
Gene Symbols: RUNX2, AML3, CBFA1, OSF2, PEBP2A
Background: The Acetylated-RUNX2 and Total-RUNX2 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human acetylated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan RUNX2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and RUNX2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-acetylation antibody is used to detect acetylated RUNX2 or biotinylated anti-RUNX2 antibody is used to detect total RUNX2 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of acetylated RUNX2 or total RUNX2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1 (CBF-alpha-1), Oncogene AML-3, Osteoblast-specific transcription factor 2 (OSF-2), Polyomavirus enhancer-binding protein 2 alpha A subunit (PEA2-alpha A, PEBP2-alpha A), SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit
-
基因ID
- 860
-
UniProt
- Q13950
抗原 See all RUNX2 ELISA试剂盒
-
