RPS6 ELISA 试剂盒
The 人 和 小鼠 RPS6 ELISA Kit (ABIN8070655) is a Colorimetric ELISA Kit designed to quantify 人 和 小鼠 RPS6.
产品编号 ABIN8070655
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for RPS6 ELISA 试剂盒 (ABIN8070655)
抗原
See all RPS6 ELISA试剂盒
RPS6
(Ribosomal Protein S6 (RPS6))
抗原表位
pSer6, pSer240, total
适用
人, 小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Lysate, Tissue Lysate
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原理
- Phospho-RPS6 (S240/244) and Total RPS6 ELISA Kit is a convenient and sensitive assay that can monitor the activation or function of important biological pathways in human, mouse cell lysates.
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Analytical Method
- Semi-Quantitative
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特异性
- The antibody pair provided in this kit recognizes human and mouse RPS6 phosphorylated at site Serine-240/244.
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组件
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- Pre-Coated 96-well Strip Microplate
- Positive Control
- Anti-Phospho Detection Antibody
- Anti-Pan Detection Antibody (*not included in phospho-only version)
- HRP-Conjugated Secondary Antibody
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Shaker
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare the positive control or sample dilutions
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and positive control as instructed.
- Add 100 μL positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- RPS6 (Ribosomal Protein S6 (RPS6))
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别名
- RPS6
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背景
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Gene Symbols: RPS6, OK/SW-cl.2
Protein Name (Synonyms): Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33
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基因ID
- 6194
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UniProt
- P62753
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途径
- Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
抗原 See all RPS6 ELISA试剂盒
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