REG3A ELISA 试剂盒
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北京 101111
Quick Overview for REG3A ELISA 试剂盒 (ABIN8070631)
抗原
See all REG3A ELISA试剂盒适用
检测方法
实验类型
应用范围
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原理
- Rat Reg3A ELISA Kit.
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Analytical Method
- Quantitative
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特异性
- The antibody pair provided in this kit recognizes Bovine ASAHL.
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Regenerating Islet-Derived 3 alpha (REG3A) ELISA Kit
REG3A 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Regenerating Islet-Derived 3 alpha (REG3A) ELISA KitREG3A 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Regenerating Islet-Derived 3 alpha (REG3A) ELISA KitREG3A 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Regenerating Islet-Derived 3 alpha (REG3A) ELISA KitREG3A 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Regenerating Islet-Derived 3 alpha (REG3A) ELISA KitREG3A 适用: 猴 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Regenerating Islet-Derived 3 alpha (REG3A) ELISA KitREG3A 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Regenerating Islet-Derived 3 alpha (REG3A) ELISA KitREG3A 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Regenerating Islet-Derived 3 alpha (REG3A) ELISA KitWide Range REG3A 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Regenerating Islet-Derived 3 alpha (REG3A) ELISA KitREG3A 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Regenerating Islet-Derived 3 alpha (REG3A) ELISA KitREG3A 适用: 人 AA 27-175 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma (heparin), Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- REG3A (Regenerating Islet-Derived 3 alpha (REG3A))
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别名
- Reg3
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背景
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Gene Symbols: Reg3a
Protein Name (Synonyms): Regenerating islet-derived protein 3-alpha, REG-3-alpha, Islet of Langerhans regenerating protein 3, REG 3, Lithostathine 3, Pancreatitis-associated protein 2, RegIII, Regenerating islet-derived protein III-alpha, Reg III-alpha
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UniProt
- P35231
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