RORA ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 RORA.
产品编号 ABIN8070609
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for RORA ELISA 试剂盒 (ABIN8070609)
抗原
See all RORA ELISA试剂盒
RORA
(RAR-Related Orphan Receptor A (RORA))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
1.48 ng/mL - 500 ng/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma, Serum
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最低检测浓度
- 1.48 ng/mL
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原理
- The Human RORA ELISA kit is a pre-coated sandwich-based assay kit designed to quantitatively measure RORA levels in human cell culture media, plasma, and serum.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human RORA. Other species not determined.
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组件
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- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
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RAR-Related Orphan Receptor A (RORA) ELISA Kit
RORA 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- RORA (RAR-Related Orphan Receptor A (RORA))
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别名
- RORA
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背景
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Gene Symbols: RORA
Protein Name (Synonyms): Nuclear receptor ROR-alpha (Nuclear receptor RZR-alpha) (Nuclear receptor subfamily 1 group F member 1) (RAR-related orphan receptor A) (Retinoid-related orphan receptor-alpha)
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基因ID
- 6095
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UniProt
- P35398
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途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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