PRKAA1 ELISA 试剂盒
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北京 101111
Quick Overview for PRKAA1 ELISA 试剂盒 (ABIN8070559)
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适用
检测方法
实验类型
应用范围
样品类型
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原理
- Phospho-AMPKa1 (T183) and Total AMPKa1 ELISA Kit is a convenient and sensitive assay that can monitor the activation or function of important biological pathways in human, mouse, rat cell lysates.
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Analytical Method
- Semi-Quantitative
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特异性
- The antibody pair provided in this kit recognizes human, mouse and rat AMPK subunit alpha-1 phosphorylated at site Threonine-183.
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组件
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- Pre-Coated 96-well Strip Microplate
- Positive Control
- Anti-Phospho Detection Antibody
- Anti-Pan Detection Antibody (*not included in phospho-only version)
- HRP-Conjugated Secondary Antibody
- HRP-Streptavidin (*not included in phospho-only version)
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Shaker
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare the positive control or sample dilutions
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Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA Kit
PRKAA1 适用: 人, 小鼠, 大鼠 pSer487 Colorimetric Sandwich ELISA Cell Culture Lysate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 人, 小鼠, 大鼠 pSer487 Colorimetric Sandwich ELISA Cell Culture Lysate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 人 Colorimetric Competition ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) ELISA KitPRKAA1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and positive control as instructed.
- Add 100 μL positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
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别名
- AMPKa1
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背景
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Gene Symbols: PRKAA1, AMPK1
Protein Name (Synonyms): 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK subunit alpha-1), Acetyl-CoA carboxylase kinase (ACACA kinase), Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase), Tau-protein kinase PRKAA1
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基因ID
- 556210578765248
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UniProt
- Q13131, Q5EG47, P54645
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途径
- AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect
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