Peroxiredoxin 6 ELISA 试剂盒
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Quick Overview for Peroxiredoxin 6 ELISA 试剂盒 (ABIN8070377)
抗原
See all Peroxiredoxin 6 (PRDX6) ELISA试剂盒抗原表位
适用
检测方法
实验类型
应用范围
样品类型
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原理
- Measures sumoylated Peroxiredoxin 6 (PRDX6) and total Peroxiredoxin 6 in human cell and tissue lysates, enabling precise assessment of Peroxiredoxin 6 sumoylation by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.
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Analytical Method
- Quantitative
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组件
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- Size / Qty
- Microplate Coated With Anti-Pan-Peroxiredoxin 6 Antibody
- Anti-SUMOylation Detection Antibody
- Anti-Peroxiredoxin 6 Detection Antibody
- HRP-Conjugated Streptavidin
- 200 μL
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare sample dilutions
- Distilled or deionized water
- Shaker
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Peroxiredoxin 6 (PRDX6) ELISA Kit
PRDX6 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Peroxiredoxin 6 (PRDX6) ELISA KitPRDX6 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxiredoxin 6 (PRDX6) ELISA KitPRDX6 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Peroxiredoxin 6 (PRDX6) ELISA KitPRDX6 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Peroxiredoxin 6 (PRDX6) ELISA KitPRDX6 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxiredoxin 6 (PRDX6) ELISA KitPRDX6 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Peroxiredoxin 6 (PRDX6) ELISA KitPRDX6 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed.
- Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- Peroxiredoxin 6 (PRDX6)
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别名
- Peroxiredoxin-6
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背景
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Gene Symbols: PRDX6, AOP2, KIAA0106
Background: The SUMOylated-Peroxiredoxin 6 and Total-Peroxiredoxin 6 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human sumoylated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan Peroxiredoxin 6 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Peroxiredoxin 6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-sumoylation antibody is used to detect sumoylated Peroxiredoxin 6 or biotinylated anti-Peroxiredoxin 6 antibody is used to detect total Peroxiredoxin 6 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of sumoylated Peroxiredoxin 6 or total Peroxiredoxin 6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Peroxiredoxin-6, 1-Cys peroxiredoxin (1-Cys PRX), 24 kDa protein, Acidic calcium-independent phospholipase A2 (aiPLA2), Antioxidant protein 2, Glutathione-dependent peroxiredoxin, Liver 2D page spot 40, Lysophosphatidylcholine acyltransferase 5 (LPC acyltransferase 5, LPCAT-5, Lyso-PC acyltransferase 5), Non-selenium glutathione peroxidase (NSGPx), Red blood cells page spot 12
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基因ID
- 9588
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UniProt
- P30041
抗原 See all Peroxiredoxin 6 (PRDX6) ELISA试剂盒
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