Nucleoprotein ELISA 试剂盒
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Quick Overview for Nucleoprotein ELISA 试剂盒 (ABIN8070297)
抗原
适用
检测方法
实验类型
应用范围
样品类型
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原理
- SFTS Virus Nucleoprotein (NP) Human IgA ELISA kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgA autoantibody against NP in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.
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Analytical Method
- Quantitative
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组件
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- Pre-coated 96-well Microplate
- Wash Buffer
- Positive Control
- Biotinylated Secondary Antibody
- HRP-Streptavidin Concentrate
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent
- Sample Diluent
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL Positive Control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Biotinylated Secondary Antibody into respective wells. Incubate 1 hour at room temperature.
- Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- Nucleoprotein
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物质类
- Influenza Protein
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背景
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Background: This < span class="italic-txt">in-vitro ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates are coated with the pathogen antigen, which combines with the corresponding antibody present in a sample and positive control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA/G/M antibody is added, a complex forms between the biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-pathogen antigen antibody present. The stop solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.
Protein Name (Synonyms): Nucleoprotein, Nucleocapsid protein
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基因ID
- 13231122
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UniProt
- F1BA48
抗原
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