Nuclease ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of Serratia marcescens Nuclease.
产品编号 ABIN8070291
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Nuclease ELISA 试剂盒 (ABIN8070291)
抗原
See all Nuclease products
Nuclease
适用
Serratia marcescens
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
19.92 pg/mL - 9600 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant
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最低检测浓度
- 19.92 pg/mL
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原理
- Nuclease ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of nucleases, including SuperNuclease® and Benzonase®, in cell culture supernatants and purified biological products while nuclease is supplied to remove host cell DNA during manufacturing.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit has been validated with SuperNuclease®, Benzonase® and RayBio® Universal Nuclease, and should react with other Serratia marcescens nuclease products but not tested. Both non-denatured and denatured nuclease can be detected by this kit.
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组件
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- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard and sample dilutions
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AccuSignal™ Nuclease ELISA Kit for Impurity Detection
ELISA, Imp De Colorimetric Quantitative
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- Nuclease
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背景
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Gene Symbols: nucA nuc
Protein Name (Synonyms): Nuclease (EC 3.1.30.2) (Endonuclease) [Cleaved into: Nuclease isoform Sm2, Nuclease isoform Sm3, Nuclease isoform Sm1
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UniProt
- P13717
抗原 See all Nuclease products
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