NOS2 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for NOS2 ELISA 试剂盒 (ABIN8070258)
抗原
See all NOS2 ELISA试剂盒抗原表位
适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- Measures citrullinated iNOS and total iNOS in human cell and tissue lysates, enabling precise assessment of iNOS citrullination by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.
-
Analytical Method
- Quantitative
-
组件
-
- Size / Qty
- Microplate Coated With Anti-Pan-iNOS Antibody
- Anti-Citrullination Detection Antibody
- Anti-iNOS Detection Antibody
- HRP-Conjugated Streptavidin
- 200 μL
- Wash Buffer
- Assay Diluent
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
-
试剂未包括
-
- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare sample dilutions
- Distilled or deionized water
- Shaker
-
-
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit
NOS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 IU/mL - 50 IU/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 兔 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nitric Oxide Synthase 2, Inducible (NOS2) ELISA KitNOS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents and samples as instructed.
- Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
-
-
- NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))
-
别名
- iNOS
-
背景
-
Gene Symbols: NOS2, NOS2A
Background: The Citrullinated-iNOS and Total-iNOS ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human citrullinated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan iNOS antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and iNOS present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-citrullination antibody is used to detect citrullinated iNOS or biotinylated anti-iNOS antibody is used to detect total iNOS . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of citrullinated iNOS or total iNOS bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Nitric oxide synthase, inducible, Hepatocyte NOS (HEP-NOS), Inducible NO synthase (Inducible NOS, iNOS), NOS type II, Peptidyl-cysteine S-nitrosylase NOS2
-
基因ID
- 4843
-
UniProt
- P35228
-
途径
- Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原 See all NOS2 ELISA试剂盒
-
