NPY ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for NPY ELISA 试剂盒 (ABIN8070236)
抗原
See all NPY ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.1 ng/mL
-
原理
- Human/Mouse/Rat Pro-Neuropeptide Y/CPON EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA
-
Analytical Method
- Quantitative
-
特异性
- This kit was designed to recognize the C-terminus of pro-NPY and therefore will detect full-length pro-NPY and CPON. However, it does not recognize active forms including NPY 1-36, 2-36, 3-36 or 3-35. Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II and APC.
-
灵敏度
- 0.19 ng/mL
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
-
-
Neuropeptide Y (NPY) ELISA Kit
NPY 适用: 人 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 大鼠 Colorimetric Competition ELISA 2.47 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 人 Colorimetric Competition ELISA 4.94 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 小鼠 Colorimetric Competition ELISA 2.47 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: Cow Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Neuropeptide Y (NPY) ELISA KitNPY 适用: 小鼠 Colorimetric Sandwich ELISA 1.87 pg/mL - 120 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Neuropeptide Y (NPY) ELISA KitNPY 适用: 人 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- Pro-NPY Standard, Biotinylated Pro-NPY peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
-
-
- NPY (Neuropeptide Y (NPY))
-
别名
- CPON
-
背景
-
Gene Symbols: NPY
Background: Neuropeptide Y (NPY) is a 36 amino acid peptide hormone found in the neural system and has an important role in obesity.
The main effect of Neuropeptide Y is increased food intake and decreased physical activity. It also increases the proportion of energy stored as fat and blocks nociceptive signals to the brain. In addition to its role in obesity, Neuropeptide Y has been associated with a number of other physiologic processes in the brain, including the regulation of energy balance, memory and learning, and epilepsy.
Animal studies strongly demonstrate that the stimulation of Neuropeptide Yergic activity via the administration of certain Neuropeptide Y agonists increases food intake compared to control animals. The effects of Neuropeptide Yergic activity on food intake is also demonstrated by the blockade of certain Neuropeptide Y receptors (Y1 and Y5 receptors), which expectedly inhibited Neuropeptide Yergic activity, thus, decreases food intake. For its role in obesity, an increase in Neuropeptide Y is caused by high levels of glucocorticosteriods through directly activating type II glucocorticosteriods receptors and indirectly, by abolishing the negative feedback of CRF on Neuropeptide Y synthesis and release. Meanwhile, obesity-induced insulin resistance and the mutation of the leptin receptor (ObRb) results in the abolishment of other negative feedback mechanisms to regulate Neuropeptide Yergic activity and ultimately food intake. High levels of Neuropeptide Y were also found in the cerebrospinal fluid of patients with anorexia nervosa.
The 291 bp coding sequence of the NPY gene synthesizes the pre-pro form of NPY, a 97 aa precursor peptide (see diagram below). Cleavage of the n-terminal 29 aa results in pro-NPY (69 aa), which then undergoes a further cleavage, yielding NPY 1-39 along with the 30-aa C-terminal peptide known as C-flanking peptide of NPY (CPON). NPY 1-39 is further processed to the mature form (NPY 1-36). The function of CPON is not currently understood.Protein Name (Synonyms): Pro-neuropeptide Y [Cleaved into: Neuropeptide Y (Neuropeptide tyrosine) (NPY) C-flanking peptide of NPY (CPON)]
-
基因ID
- 4852
-
UniProt
- P01303
-
途径
- Feeding Behaviour
抗原 See all NPY ELISA试剂盒
-
